High Pressure Freezing of Cyanobacteria for Immunolocalization Studies
نویسندگان
چکیده
Cyanobacteria are unicellular photosynthetic prokaryotes. The enzymes of the Calvin cycle of photosynthesis are known to be randomly distributed in the cytosol of these organisms. Such random location of these soluble enzymes, is in disparity with their precise function and regulation for photosynthetic carbon assimilation. This location has been conjectured from the fact that they can be isolated in soluble form. Immunoelectron microscopy can aid in deciphering their exact intracellular location. However, conventional electron microscopy suffers from Abstract
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